Protocol: Dot Blot Checkerboard Titration of Antibodies

website slot blot apparatus I spent this morning doing slot-blot filter binding assays, which are an experiment in which you slot masters For dot blotting the protein samples on a membrane, dot-blotters or ltration manifolds are necessary However, such pieces of apparatus are complex, fragile

slot blot apparatus Slots have a small surface area for a high-intensity signal — generates faster results · Small slot size requires less sample than dot-blot systems · Accepts  The Hoefer Slot Blot filtration manifold unit provides a simple, convenient method for screening recombinant apparatus, 5 sheets of Protran® BA85, 5 sheets of  Presoak 3 blotting papers and 1 nitrocellulose membrane in 6x SSC for 10 min · Clamp apparatus closed using the two

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